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多肉植物离体再生研究进展
卫俨1,2,3,李梅兰3,朱木兰1,2
1.中国科学院 上海辰山植物科学研究中心,上海 201602;2.中国科学院上海生命科学研究院 植物生理生态研究所,上海 200032;3.山西农业大学 园艺学院,山西 太谷 030801
摘要:
通过品种、外植体类型、培养基、外源植物激素的选择等几方面对国内多肉植物的离体再生研究现状进行了综述。发现多肉植物的离体再生研究多以种子、叶片、茎段、花器官等为外植体;不定芽诱导阶段常用的培养基类型为MS培养基,不定根诱导常用1/2 MS培养基;6?苄氨基腺嘌呤(6?BA)、萘乙酸(NAA)和激动素(KT)作为常见的外源植物激素,被广泛运用于多肉植物愈伤组织诱导、不定芽诱导、不定根诱导等离体再生各阶段;分析了当前存在的问题和发展前景,以期为日后多肉植物的相关研究提供可行性建议。
关键词:  多肉植物  离体再生  研究进展
DOI:10.14188/j.ajsh.2018.04.003
分类号:S682.33
基金项目:
Research progress in in vitro regeneration of succulent plants
WEI Yan1,2,3,LI Meilan3,ZHU Mulan1,2
1.Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China;2.Institute of Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;3.College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
Abstract:
The achievements in recent years in in vitro regeneration of succulent plants are reviewed, including the selection of variety, explant, culture medium and the uses of plant hormones. Seeds, leaves, stem segments, flower organs are often adopted in succulent plant tissue culture; the type of media commonly used in the induction of adventitious buds is MS medium, adventitious root induction commonly uses 1/2 MS medium; 6?benzyl amino adenine (6?BA), 1?naphthylacetic acid (NAA) and kinetin (KT), as common exogenous plant hormones, are widely used in callus induction, adventitious bud induction, adventitious root induction and other regeneration stages in vitro of succulent plants; The current problems and development prospects are analysed in order to provide feasible suggestions for related research on succulents.
Key words:  succulent plant  in vitro regeneration  research progress