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基于珙桐SNP位点的dCAPS标记开发
许瑾1,高妍1,杨碧2,宋云1,李明福1,陈乃中1
1.中国检验检疫科学研究院,北京 100029;2.昆明海关,云南 昆明 650228
摘要:
本研究收集了珙桐、喜树等19份植物材料,运用分子生物学和生物信息学手段比对分析了rpoB,rpoC,matK等8个基因碱基序列上的单核苷酸多态性(SNP)位点,在atpF?atpH基因上找到了合适的SNP位点并设计了dCAPS引物,经PCR扩增和酶切验证后,将SNP转化为衍生型酶切扩增多态性序列(dCAPS)标记,可促进SNP标记在珙桐的遗传图谱构建、基因定位、种质资源鉴定、遗传多样性研究等领域的应用。
关键词:  单核苷酸多态性(SNP)  内切酶  珙桐  衍生型酶切扩增多态性序列(dCAPS)
DOI:10.14188/j.ajsh.2018.04.006
分类号:Q789
基金项目:国家重点研发计划(2017YFF0210300)
Exploration of SNP and relative dCAPS markers in Davidia involucrata
XU Jin1,GAO Yan1,YANG Bi2,SONG Yun1,LI Mingfu1,CHEN Naizhong1
1.Chinese Academy of Inspection and Quarantine, Beijing 100029, China;2.Kunming Customs, Kunming 650228, Yunnan, China
Abstract:
Nineteen plant materials (Davidia inbolucrata, Camptotheca acuminate, etc.) were collected, and nucleotide sequences of eight gene such as rpoB, rpoC, matK were analyzed by molecular biology and bioinformatics software to search for single nucleotide polymorphism (SNP). The derived cleaved amplified polymorphic sequence (dCAPS) primers were designed based on the SNPs and the genomic DNA was used for amplification. The amplicons were digested by restrict enzymes to convert SNPs into dCAPS markers which can be applied in genetic map construction, gene mapping, germplasm identification, genetic diversity research and other fields.
Key words:  single nucleotide polymorphism (SNP)  endonuclease  Davidia involucrata  derived cleaved amplified polymorphic sequence (dCAPS)