引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 132次   下载 70 本文二维码信息
码上扫一扫!
分享到: 微信 更多
高产木质素酶荧光假单胞杆菌L⁃520的筛选及其发酵工艺优化
石晓玲1,林胜红1,刘震飞1,潘晓梅1,ALI Ihsan1,田永强1
兰州交通大学 化学与生物工程学院,甘肃 兰州 730070
摘要:
利用苯胺蓝鉴别培养基及产酶培养基进行菌种筛选,从甘肃兴隆山分离得到的10株菌株中筛选到一株高产木质素酶活力的菌株L?520,对该菌株进行16S rDNA鉴定,确定该菌为荧光假单胞杆菌(Pseudomonas fluorescens)。分别考察了接种量、装液量,初始pH对L?520发酵产酶的影响,以及碳源、氮源对木质素酶活力的影响。结果得到最佳培养基为:蔗糖 1%,NH4Cl 0.2%, KH2PO4 0.1%,MgSO4?7H2O 0.05%。优化后的发酵条件为:初始pH 5,接种量3%,装液量50%。经发酵工艺优化后,漆酶(Lac)、木质素过氧化物酶(LiP)、锰过氧化物酶(MnP)三种酶活分别为16.43 U/L, 106.32 U/L, 95.89 U/L,与初始酶活相比分别提高了8.7、14.74、11.09倍。本研究筛选得到的荧光假单胞杆菌有助于染料废水中偶氮染料的降解。
关键词:  木质素酶  荧光假单胞杆菌  菌种筛选  发酵优化
DOI:10.14188/j.ajsh.2018.04.009
分类号:Q93
基金项目:
Screening of Pseudomonas fluorescens L⁃520 with high ligninase activity and optimization of its fermentation process
SHI Xiaoling1,LIN Shenghong1,LIU Zhenfei1,PAN Xiaomei1,ALI Ihsan1,TIAN Yongqiang1
School of Chemical and Biological Engineering, Lanzhou Jiaotong university, Lanzhou 730070, Gansu, China
Abstract:
A strain L?520 of high yield lignin enzyme activity was screened from 10 strains isolated from the Xinlong mountain in Gansu by aniline blue differential medium. The strain was identified as Pseudomonas fluorescens by 16S rDNA sequence analysis. The influencing factors of enzyme production fermentation of L?520, such as inoculum volume, liquid volume, initial pH were studied, and the carbon source and nitrogen source effects on the activity of lignin enzyme were also investigated. The results showed that, the optimum fermentation medium was: sucrose 1%, NH4Cl 0.2%, KH2PO4 0.1%,MgSO4·7H2O 0.05%; the optimum cultivation conditions were initial pH 5, inoculum amount 3%, liquid volume 50%. After optimization of the fermentation process, the activities of laccase(Lac), lignin peroxidase(LiP) and manganese?dependent peroxidase(MnP) were 16.43 U/L, 106.32 U/L and 95.89 U/L, respectively, which were increased 8.7, 14.74 and 11.09 times, respectively, compared with the initial enzyme activity. Pseudomonas fluorescens L?520, the high?yielding ligninase strain obtained in this study could be of great importance for the degradation of azo dyes in dye wastewater.
Key words:  ligninase  Pseudomonas fluorescens  strain screening  fermentation optimization